8EBB

Crystal structure of SIX6 from Fusarium oxysporum f. sp. lycopersici

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95336
Spacegroup name	P 21 21 2
Unit cell lengths	76.303, 93.544, 60.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.770 - 1.880
R-factor	0.1948
Rwork	0.193
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SIX6 alphafold model
RMSD bond length	0.008
RMSD bond angle	0.933
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	1.920
High resolution limit [Å]	1.880	1.880
Rmerge	0.070	1.429
Rmeas	0.082	1.668
Rpim	0.042	0.857
Number of reflections	35252	2205
<I/σ(I)>	11.8	0.9
Completeness [%]	96.8
Redundancy	6.9
CC(1/2)	0.990	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium tartrate, 20% PEG3350