8EB5
Tandem of Hermes transposase BED domain in complex with the quasi palindrome of its transposon left-end
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2020-07-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.28335 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 55.271, 55.271, 212.573 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.640 - 2.500 |
R-factor | 0.2316 |
Rwork | 0.231 |
R-free | 0.23820 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | BUSTER (2.10.4 (11-DEC-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.640 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.044 | 1.224 |
Rmeas | 0.046 | 1.224 |
Rpim | 0.011 | 0.272 |
Number of reflections | 7292 | 694 |
<I/σ(I)> | 35.47 | 2.39 |
Completeness [%] | 99.8 | 100 |
Redundancy | 19.2 | 19.23 |
CC(1/2) | 0.999 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 4000, Bis-TRIS |