8EA1
Structure of kudzu 2-hydroxyisoflavanone dehydratase in complex with P-NITROPHENOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-16 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.844, 102.844, 70.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.360 - 2.290 |
| R-factor | 0.1816 |
| Rwork | 0.181 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o7r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Rmerge | 0.054 | 0.548 |
| Number of reflections | 19522 | 1912 |
| <I/σ(I)> | 42.2 | 5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.9 | 11.1 |
| CC(1/2) | 0.860 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18% PEG3350, 0.1 M HEPES pH7.5 |
