8E9T

Crystal structure of wild-type E. coli aspartate aminotransferase in the ligand-free form at 303 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	303
Detector technology	PIXEL
Collection date	2021-06-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1158
Spacegroup name	P 63
Unit cell lengths	143.830, 143.830, 81.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	124.560 - 2.130
R-factor	0.1744
Rwork	0.172
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x28
RMSD bond length	0.002
RMSD bond angle	0.504
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	124.950	2.170
High resolution limit [Å]	2.130	2.130
Number of reflections	53790	2679
<I/σ(I)>	6
Completeness [%]	100.0
Redundancy	5.1
CC(1/2)	0.993	0.263

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	HEPES, ammonium sulfate, PEG 400, maleate