8E9A
Crystal structure of AsfvPolX in complex with 10-23 DNAzyme and Mg
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.829, 48.419, 139.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.920 - 2.690 |
| R-factor | 0.2566 |
| Rwork | 0.255 |
| R-free | 0.28860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xm8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.159 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 139.850 | 2.820 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 21227 | 2818 |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 97.5 | 99.7 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.977 | 0.305 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.2 M magnesium chloride, 0.1 M HEPES (pH 7.5), and 25% PEG-3350 |
