8E9A
Crystal structure of AsfvPolX in complex with 10-23 DNAzyme and Mg
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.829, 48.419, 139.845 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.920 - 2.690 |
R-factor | 0.2566 |
Rwork | 0.255 |
R-free | 0.28860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xm8 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.159 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 139.850 | 2.820 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 21227 | 2818 |
<I/σ(I)> | 2.1 | |
Completeness [%] | 97.5 | 99.7 |
Redundancy | 3.4 | |
CC(1/2) | 0.977 | 0.305 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.2 M magnesium chloride, 0.1 M HEPES (pH 7.5), and 25% PEG-3350 |