8E8P
GSTZ1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-06-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.541780 |
Spacegroup name | P 1 |
Unit cell lengths | 42.056, 50.079, 54.645 |
Unit cell angles | 83.47, 69.76, 73.68 |
Refinement procedure
Resolution | 26.682 - 2.281 |
R-factor | 0.2544 |
Rwork | 0.252 |
R-free | 0.29560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fw1 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.280 | 6.240 | 2.300 |
Rmerge | 0.071 | 0.037 | 0.492 |
Rmeas | 0.087 | 0.045 | 0.611 |
Rpim | 0.050 | 0.026 | 0.358 |
Number of reflections | 17447 | 891 | 865 |
<I/σ(I)> | 13.7 | ||
Completeness [%] | 96.3 | 98.9 | 94.7 |
Redundancy | 3 | 2.8 | 3 |
CC(1/2) | 0.996 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MgCl2; 0.1M alpha-D-galacturonic acid, pH 6.5; 12% w/v PEG6000 |