8E8J

Human DNA polymerase eta-DNA-rG-ended primer-dGMPNPP ternary mismatch complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-02-08
Detector	RAYONIX MX-300
Wavelength(s)	0.978720
Spacegroup name	P 61
Unit cell lengths	98.019, 98.019, 81.849
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.010 - 2.400
R-factor	0.1934
Rwork	0.192
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.010	49.010	2.540
High resolution limit [Å]	2.400	7.120	2.400
Rmerge	0.110	0.031	0.765
Rmeas	0.116	0.033	0.854
Total number of observations	344980
Number of reflections	34497	1304	5579
<I/σ(I)>	21.92	68.28	2.39
Completeness [%]	99.8	99.8	99.1
Redundancy	10	11.248	5.035
CC(1/2)	0.998	1.000	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES