8E8G

Human DNA polymerase eta-DNA-rU-ended primer ternary mismatch complex:reaction with 10 mM Mn2+ for 180s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.976180
Spacegroup name	P 61
Unit cell lengths	98.060, 98.060, 81.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.100 - 2.130
R-factor	0.2033
Rwork	0.201
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.100	32.100	2.100
High resolution limit [Å]	2.050	9.160	2.050
Rmerge	0.081	0.052	1.256
Rmeas	0.084	0.053	1.536
Total number of observations	544101
Number of reflections	52050	615	1992
<I/σ(I)>	13.93	51.36	0.8
Completeness [%]	94.8	99.5	49.8
Redundancy	10.453	20.007	2.799
CC(1/2)	0.999	0.999	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES