8E8F

Human DNA polymerase eta-DNA-rU-ended primer ternary mismatch complex:reaction with 10 mM Mn2+ for 120s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.976180
Spacegroup name	P 61
Unit cell lengths	98.236, 98.236, 81.296
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.160 - 2.140
R-factor	0.2036
Rwork	0.200
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.160	32.160	2.250
High resolution limit [Å]	2.120	6.330	2.120
Rmerge	0.048	0.033	0.460
Rmeas	0.051	0.034	0.517
Total number of observations	454789
Number of reflections	48711	1863	7232
<I/σ(I)>	22.69	65.28	2.29
Completeness [%]	98.5	99.7	90.7
Redundancy	9.336	10.275	4.719
CC(1/2)	1.000	0.999	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES