8E8E

Human DNA polymerase eta-DNA-rU-ended primer ternary mismatch complex:reaction with 10 mM Mn2+ for 90s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.976180
Spacegroup name	P 61
Unit cell lengths	98.480, 98.480, 81.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.430 - 2.050
R-factor	0.2109
Rwork	0.209
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.430	28.430	2.110
High resolution limit [Å]	2.050	9.180	2.050
Rmerge	0.100	0.064	0.490
Rmeas	0.103	0.065	0.606
Total number of observations	735591
Number of reflections	52663	617	2102
<I/σ(I)>	17.09	42.9	1.76
Completeness [%]	95.3	99.5	51.6
Redundancy	13.968	22.681	2.659
CC(1/2)	0.999	0.999	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES