8E8D

Human DNA polymerase eta-DNA-rU-ended primer ternary mismatch complex:reaction with 10 mM Mn2+ for 60s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.976180
Spacegroup name	P 61
Unit cell lengths	98.480, 98.480, 81.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.430 - 2.090
R-factor	0.2015
Rwork	0.200
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.430	31.430	2.110
High resolution limit [Å]	2.050	9.180	2.050
Rmerge	0.750	0.484	0.498
Rmeas	0.764	0.493	0.614
Total number of observations	687643
Number of reflections	52662	616	2102
<I/σ(I)>	16.87	47.4	1.71
Completeness [%]	95.3	99.4	51.6
Redundancy	13.058	22.485	2.659
CC(1/2)	0.999	0.999	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES