8E8C

Human DNA polymerase eta-DNA-rU-ended primer ternary mismatch complex:reaction with 10 mM Mn2+ for 30s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.976180
Spacegroup name	P 61
Unit cell lengths	97.800, 97.800, 81.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.010 - 2.250
R-factor	0.1937
Rwork	0.192
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.010	32.010	2.100
High resolution limit [Å]	2.050	9.150	2.050
Rmerge	0.069	0.038	1.178
Rmeas	0.070	0.039	1.320
Total number of observations	733315
Number of reflections	48522	615	1181
<I/σ(I)>	21.85	74.65	1.02
Completeness [%]	88.0	99.2	29.1
Redundancy	15.113	22.976	4.414
CC(1/2)	1.000	1.000	0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES