8E89

Human DNA polymerase eta-DNA-rU-ended primer-binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127130
Spacegroup name	P 61
Unit cell lengths	97.394, 97.394, 80.911
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.400 - 2.200
R-factor	0.2038
Rwork	0.201
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.400	37.400	2.330
High resolution limit [Å]	2.200	6.530	2.200
Rmerge	0.081	0.036	0.853
Rmeas	0.085	0.038	0.896
Total number of observations	451872
Number of reflections	43810	1663	7040
<I/σ(I)>	15.8	50.99	2.01
Completeness [%]	99.8	99.8	99
Redundancy	10.314	11.184	10.762
CC(1/2)	0.999	0.999	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES