8E88

Human DNA polymerase eta-DNA-rU-ended primer-dGMPNPP ternary mismatch complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-02-08
Detector	RAYONIX MX-300
Wavelength(s)	0.978720
Spacegroup name	P 61
Unit cell lengths	98.347, 98.347, 81.171
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.590 - 2.400
R-factor	0.1964
Rwork	0.195
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.590	42.590	2.540
High resolution limit [Å]	2.400	7.100	2.400
Rmerge	0.068	0.023	0.557
Rmeas	0.075	0.025	0.648
Total number of observations	183984
Number of reflections	34084	1315	5165
<I/σ(I)>	19.67	56.67	2.21
Completeness [%]	98.9	99.7	93.2
Redundancy	5.398	5.69	3.521
CC(1/2)	0.999	1.000	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES