8E87

Human DNA polymerase eta-DNA-rA-ended primer-dGMPNPP ternary mismatch complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-03-18
Detector	RAYONIX MX-300
Wavelength(s)	0.978720
Spacegroup name	P 61
Unit cell lengths	97.728, 97.728, 81.881
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.320 - 2.190
R-factor	0.2021
Rwork	0.200
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.320	42.320	2.320
High resolution limit [Å]	2.190	6.490	2.190
Rmerge	0.037	0.027	0.153
Rmeas	0.041	0.030	0.198
Total number of observations	213299
Number of reflections	44645	1724	6764
<I/σ(I)>	24.84	53.44	4.64
Completeness [%]	98.6	99.7	92
Redundancy	4.778	5.686	2.128
CC(1/2)	0.999	0.999	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES