8E86
Human DNA polymerase eta-DNA-rC-ended primer-dGMPNPP ternary mismatch complex with Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.127230 |
Spacegroup name | P 61 |
Unit cell lengths | 98.380, 98.380, 82.063 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.200 - 1.780 |
R-factor | 0.1949 |
Rwork | 0.194 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7m7l |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.200 | 32.200 | 1.890 |
High resolution limit [Å] | 1.780 | 5.320 | 1.780 |
Rmerge | 0.133 | 0.060 | 1.260 |
Rmeas | 0.140 | 0.063 | 1.322 |
Total number of observations | 893092 | ||
Number of reflections | 84523 | 3178 | 13573 |
<I/σ(I)> | 14.02 | 42.2 | 2.1 |
Completeness [%] | 99.9 | 99.8 | 99.5 |
Redundancy | 10.566 | 10.938 | 10.736 |
CC(1/2) | 0.998 | 0.998 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000MME, 0.1 M MES |