8E7Z
RsTSPO mutant -A138F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.553, 99.535, 95.484 |
| Unit cell angles | 90.00, 100.12, 90.00 |
Refinement procedure
| Resolution | 47.130 - 2.600 |
| R-factor | 0.2065 |
| Rwork | 0.204 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uc1 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.371 |
| Number of reflections | 16650 | 1658 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2 | |
| CC(1/2) | 0.984 | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 26-30% PEG600, HEPES pH 7.5, 100mM Sodium Acetate, 100mM Zinc Acetate |
