8E7Z
RsTSPO mutant -A138F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 58.553, 99.535, 95.484 |
Unit cell angles | 90.00, 100.12, 90.00 |
Refinement procedure
Resolution | 47.130 - 2.600 |
R-factor | 0.2065 |
Rwork | 0.204 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uc1 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.140 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.090 | 0.371 |
Number of reflections | 16650 | 1658 |
<I/σ(I)> | 10.5 | |
Completeness [%] | 100.0 | |
Redundancy | 2 | |
CC(1/2) | 0.984 | 0.615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 26-30% PEG600, HEPES pH 7.5, 100mM Sodium Acetate, 100mM Zinc Acetate |