8E7F
Crystal structure of the autotransporter Ssp from Serratia marcescens.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537, 0.9642 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.482, 55.356, 61.883 |
| Unit cell angles | 91.52, 93.04, 102.76 |
Refinement procedure
| Resolution | 39.890 - 2.000 |
| R-factor | 0.1653 |
| Rwork | 0.163 |
| R-free | 0.20150 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.460 |
| Data scaling software | XDS (2022) |
| Phasing software | Auto-Rickshaw (2022) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.078 | 0.597 |
| Rmeas | 0.093 | 0.724 |
| Rpim | 0.050 | 0.405 |
| Number of reflections | 39914 | 5596 |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 97.1 | 93.3 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.997 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.2 M potassium iodide, 20% (w/v) PEG 3350, 0.1 M HEPES |
