8E5Y
Human PU.1 ETS-Domain Bound to d(AATAAGCGGAAGTGGG) Acetate Free at pH 5.4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.059, 60.807, 44.437 |
Unit cell angles | 90.00, 116.24, 90.00 |
Refinement procedure
Resolution | 32.600 - 1.320 |
R-factor | 0.1491 |
Rwork | 0.148 |
R-free | 0.16240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e3k |
RMSD bond length | 0.009 |
RMSD bond angle | 1.179 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.600 | 1.367 |
High resolution limit [Å] | 1.320 | 1.320 |
Rmerge | 0.044 | 0.350 |
Rmeas | 0.052 | 0.408 |
Rpim | 0.027 | 0.205 |
Number of reflections | 47335 | 4710 |
<I/σ(I)> | 15.84 | 2.9 |
Completeness [%] | 97.9 | 98.37 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.996 | 0.928 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 100 mM Bis-Tris pH 5.4, 2% PEG 3350 |