8E4T

Crystal structure of the kinase domain of RTKC8 from the choanoflagellate Monosiga brevicollis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9998
Spacegroup name	P 21 21 2
Unit cell lengths	81.059, 55.513, 60.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.550 - 1.950
R-factor	0.2064
Rwork	0.204
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ueu
RMSD bond length	0.007
RMSD bond angle	0.847
Data reduction software	XDS (Version Jan 26, 2018)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.555	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.231	3.648
Rmeas	0.235	3.758
Rpim	0.046	0.883
Number of reflections	20593	1414
<I/σ(I)>	12.4	0.9
Completeness [%]	99.9	99.3
Redundancy	26.2	17
CC(1/2)	0.998	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1M SPG (Succinic acid, sodium dihydrogen phosphate and glycine) buffer pH 6.0, 25% (w/v) PEG 1500 Mixed 1:1 Protein: 10-15mg/mL in 50mM Tris pH 8.0, 200mM NaCl, 10% Glycerol, 0.5mM TCEP.