8E4H

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGAGGAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920105
Spacegroup name	P 1 21 1
Unit cell lengths	42.989, 60.608, 44.754
Unit cell angles	90.00, 116.92, 90.00

Refinement procedure

Resolution	23.770 - 1.390
R-factor	0.1365
Rwork	0.136
R-free	0.15350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.008
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.770	1.440
High resolution limit [Å]	1.390	1.390
Rmerge	0.060	0.468
Rmeas	0.065	0.509
Rpim	0.026	0.199
Number of reflections	41199	4126
<I/σ(I)>	17.63	3.07
Completeness [%]	99.7	99.81
Redundancy	6.7
CC(1/2)	0.996	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	2% PEG 3350, 100 mM Sodium Acetate, pH 4.6