8E4H
Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGAGGAAGTGGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-18 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.920105 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.989, 60.608, 44.754 |
Unit cell angles | 90.00, 116.92, 90.00 |
Refinement procedure
Resolution | 23.770 - 1.390 |
R-factor | 0.1365 |
Rwork | 0.136 |
R-free | 0.15350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e3k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.101 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.770 | 1.440 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.060 | 0.468 |
Rmeas | 0.065 | 0.509 |
Rpim | 0.026 | 0.199 |
Number of reflections | 41199 | 4126 |
<I/σ(I)> | 17.63 | 3.07 |
Completeness [%] | 99.7 | 99.81 |
Redundancy | 6.7 | |
CC(1/2) | 0.996 | 0.944 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2% PEG 3350, 100 mM Sodium Acetate, pH 4.6 |