8E3D
ZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing CAST sequence (#11)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 6 |
| Unit cell lengths | 196.181, 196.181, 54.964 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.150 - 2.620 |
| R-factor | 0.2074 |
| Rwork | 0.206 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7n5u |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.629 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.150 | 2.710 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.182 | |
| Rpim | 0.039 | 0.706 |
| Number of reflections | 70633 | 7054 |
| <I/σ(I)> | 21.7 | 1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 22.1 | 19.1 |
| CC(1/2) | 0.398 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 20% PEG3350, 0.2M Potassium chloride |
