8E2W
Structure of CRISPR-Associated DinG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.88684 |
Spacegroup name | P 65 |
Unit cell lengths | 123.311, 123.311, 136.874 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.740 - 2.950 |
R-factor | 0.1873 |
Rwork | 0.185 |
R-free | 0.21520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Alphafold Model |
RMSD bond length | 0.002 |
RMSD bond angle | 0.518 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 1.356 | |
Rpim | 0.346 | |
Number of reflections | 24916 | 2453 |
<I/σ(I)> | 20 | 2 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 20.5 | 16 |
CC(1/2) | 0.997 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 225 mM Imidazole pH 8.0, 3.5% PEG8000, and a 30% sucrose mixture. The crystal used for structure determination comprised 1 uL (5mg/ml) protein solution to 2.6 uL of mother liquor and 0.4 uL sucrose |