8E1F

Sterile Alpha Motif Domain of Human Translocation ETS Leukemia, Non-Polymer Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 65
Unit cell lengths	61.954, 61.954, 167.574
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.690 - 2.160
Rwork	0.198
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2QAR
RMSD bond length	0.012
RMSD bond angle	1.094
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.690	2.237
High resolution limit [Å]	2.160	2.160
Rmerge	0.140	1.488
Rmeas	0.143	1.528
Rpim	0.032	0.346
Number of reflections	19209	1954
<I/σ(I)>	17.04	2.64
Completeness [%]	98.5	100
Redundancy	20.2	19.3
CC(1/2)	0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	298	200mM MgSO4 Heptahydrate, 20% w/v Polyethylene Glycol 3,350