8E11
Structure of mouse DNA polymerase Beta (PolB) mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS 3.0 MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-28 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.372, 39.898, 93.804 |
| Unit cell angles | 90.00, 91.71, 90.00 |
Refinement procedure
| Resolution | 30.920 - 1.800 |
| R-factor | 0.1963 |
| Rwork | 0.192 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zqy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.720 |
| Data reduction software | SAINT (B8.40B) |
| Data scaling software | Aimless (0.7.8) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.250 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.084 | 1.088 |
| Rmeas | 0.090 | 1.207 |
| Rpim | 0.031 | 0.515 |
| Number of reflections | 55670 | 3275 |
| <I/σ(I)> | 18.4 | 1.8 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.9 | 5.2 |
| CC(1/2) | 0.999 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 0.1 M HEPES pH 7.5, 27.5% w/v PEG3350, 1.5% v/v tacsimate pH 7.0 |
