8E10
Structure of mouse polymerase beta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER IMUS 3.0 MICROFOCUS |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-27 |
Detector | Bruker PHOTON III |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 62.725, 120.534, 69.043 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.520 - 1.650 |
R-factor | 0.1775 |
Rwork | 0.173 |
R-free | 0.21420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zqy |
RMSD bond length | 0.011 |
RMSD bond angle | 1.052 |
Data reduction software | SAINT (V8.40B) |
Data scaling software | Aimless (0.7.8) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.726 | 1.680 |
High resolution limit [Å] | 1.647 | 1.647 |
Rmerge | 0.082 | 1.031 |
Rmeas | 0.088 | 1.126 |
Rpim | 0.031 | 0.447 |
Number of reflections | 63779 | 3092 |
<I/σ(I)> | 20.4 | 1.9 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 8 | 6.3 |
CC(1/2) | 0.999 | 0.625 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287.15 | 0.1 M HEPES pH 7.5, 22.5% w/v PEG3350, 1.5% v/v Tacsimate pH 7.0 |