Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.344, 65.599, 79.686 |
| Unit cell angles | 74.50, 76.82, 72.23 |
Refinement procedure
| Resolution | 33.930 - 2.150 |
| R-factor | 0.1814 |
| Rwork | 0.179 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8e0p |
| Data reduction software | xia2 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.902 | 2.370 |
| High resolution limit [Å] | 2.144 | 2.144 |
| Rmerge | 0.087 | 0.367 |
| Rpim | 0.052 | 0.231 |
| Number of reflections | 34100 | 1705 |
| <I/σ(I)> | 11.2 | 1 |
| Completeness [%] | 91.2 | 59.3 |
| Redundancy | 3.7 | 3.5 |
| CC(1/2) | 0.992 | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.8 | 293 | 0.1 M magnesium acetate, 0.1 M sodium citrate, pH 5.8, 14% PEG5000 MME |
