8E0P
Crystal structure of mouse APCDD1 in fusion with engineered MBP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.914, 98.361, 156.513 |
| Unit cell angles | 90.00, 109.73, 90.00 |
Refinement procedure
| Resolution | 39.670 - 2.330 |
| R-factor | 0.1932 |
| Rwork | 0.192 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3set |
| Data reduction software | xia2 |
| Data scaling software | STARANISO |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.670 | 2.450 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.251 | 5.124 |
| Rpim | 0.081 | 1.749 |
| Number of reflections | 134251 | 6708 |
| <I/σ(I)> | 7 | 0.4 |
| Completeness [%] | 90.5 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.995 | 0.229 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.2 M ammonium citrate, pH 7.0, 20% PEG3350, 4% NDSB-256, 5% glycerol |
