8E0P
Crystal structure of mouse APCDD1 in fusion with engineered MBP
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 123.914, 98.361, 156.513 |
Unit cell angles | 90.00, 109.73, 90.00 |
Refinement procedure
Resolution | 39.670 - 2.330 |
R-factor | 0.1932 |
Rwork | 0.192 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3set |
Data reduction software | xia2 |
Data scaling software | STARANISO |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.670 | 2.450 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.251 | 5.124 |
Rpim | 0.081 | 1.749 |
Number of reflections | 134251 | 6708 |
<I/σ(I)> | 7 | 0.4 |
Completeness [%] | 90.5 | |
Redundancy | 10.6 | |
CC(1/2) | 0.995 | 0.229 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.2 M ammonium citrate, pH 7.0, 20% PEG3350, 4% NDSB-256, 5% glycerol |