8E0O
Heterotrimeric variant of tcTRP9, hetBGL03-15-18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-18 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.957, 61.662, 73.691 |
Unit cell angles | 90.00, 100.19, 90.00 |
Refinement procedure
Resolution | 29.460 - 2.100 |
R-factor | 0.2387 |
Rwork | 0.237 |
R-free | 0.26240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | computationally generated |
Data reduction software | Aimless |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.460 | 29.460 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.057 | 0.013 | 0.354 |
Rmeas | 0.060 | 0.015 | 0.374 |
Rpim | 0.019 | 0.006 | 0.118 |
Total number of observations | 301984 | 2625 | 24709 |
Number of reflections | 30580 | 422 | 2486 |
<I/σ(I)> | 27.1 | 67.7 | 6 |
Completeness [%] | 100.0 | 97.6 | 100 |
Redundancy | 9.9 | 6.2 | 9.9 |
CC(1/2) | 1.000 | 1.000 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) PEG3350, 0.1M Bis-Tris pH 6.5, 0.2 M. Magnesium Chloride |