8E0L
Homotrimeric variant of tcTRP9, BGL06
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.249, 78.885, 58.664 |
Unit cell angles | 90.00, 118.29, 90.00 |
Refinement procedure
Resolution | 43.210 - 2.100 |
R-factor | 0.2134 |
Rwork | 0.210 |
R-free | 0.25590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | computationally generated |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.061 | 0.054 | 0.132 |
Rmeas | 0.067 | 0.059 | 0.143 |
Rpim | 0.026 | 0.024 | 0.054 |
Total number of observations | 176681 | ||
Number of reflections | 27416 | 1424 | 1363 |
<I/σ(I)> | 18.3 | ||
Completeness [%] | 99.8 | 99.7 | 99.5 |
Redundancy | 6.4 | 6.2 | 6.7 |
CC(1/2) | 0.998 | 0.991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) PEG-1000 phosphate-citrate pH 4.2 200 mM Li2SO4 |