8E0E

nbF3:CaV beta subunit 2a complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.115830
Spacegroup name	P 65
Unit cell lengths	144.525, 144.525, 63.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.310 - 2.000
R-factor	0.1986
Rwork	0.197
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v2p
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.310	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.074
Number of reflections	51357	5099
<I/σ(I)>	32.4
Completeness [%]	99.9	99.96
Redundancy	20
CC(1/2)	1.000	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M HEPES pH7, 30%-40% MPD