8DYF
IL17A homodimer bound to Compound 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 65 |
Unit cell lengths | 96.260, 96.260, 51.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.340 - 2.020 |
R-factor | 0.2161 |
Rwork | 0.214 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hr9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.129 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.400 | 2.056 |
High resolution limit [Å] | 2.000 | 2.022 |
Number of reflections | 18098 | 914 |
<I/σ(I)> | 16.7 | |
Completeness [%] | 100.0 | |
Redundancy | 10 | |
CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290.15 | 3.2M NaCl, 0.1M Tris, pH 8.5 |