8DY0
Crystal Structure of spFv GLK1 HL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 37.940, 50.740, 63.200 |
Unit cell angles | 76.35, 89.20, 69.16 |
Refinement procedure
Resolution | 35.350 - 2.100 |
R-factor | 0.1842 |
Rwork | 0.182 |
R-free | 0.22380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3na9 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.350 | 35.350 | 2.150 |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.152 | 0.100 | 0.607 |
Rmeas | 0.167 | 0.109 | 0.721 |
Total number of observations | 132954 | ||
Number of reflections | 24805 | 279 | 1840 |
<I/σ(I)> | 7.38 | 16.89 | 2.33 |
Completeness [%] | 99.5 | 99.6 | 99.2 |
Redundancy | 5.36 | 6.082 | 3.386 |
CC(1/2) | 0.991 | 0.990 | 0.828 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 100 mM MES, 750 mM (NH4)2SO4, 5% PEG 400 |