8DWN

Crystal structure of bis-phosphorylated insulin receptor kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-10-09
Detector	RIGAKU RAXIS II
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	57.943, 70.189, 88.775
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.640 - 2.150
R-factor	0.1954
Rwork	0.190
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1irk
RMSD bond length	0.010
RMSD bond angle	0.969
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.640	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.048	0.148
Rpim	0.036	0.119
Number of reflections	18050	1788
<I/σ(I)>	14.78	6.83
Completeness [%]	88.9	89.8
Redundancy	2.3	2
CC(1/2)	0.996	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	25% PEG 6000, 0.2 M malate-imidazole