8DW0
Glycosylase MutY variant N146S in complex with DNA containing d(8-oxo-G) paired with an enzyme-generated abasic site (AP) product and crystallized with sodium acetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.11589 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.940, 86.560, 142.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.020 - 1.680 |
| R-factor | 0.2006 |
| Rwork | 0.200 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8dvp |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.020 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Number of reflections | 101188 | 7614 |
| <I/σ(I)> | 0.0601 | |
| Completeness [%] | 97.7 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.997 | 0.012 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.13 | PEG 4000, sodium acetate, Tris.HCl |
