8DVZ

Structure of the Campylobacter concisus glycosyltransferase PglA R282V variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	97.971, 97.971, 100.768
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.900 - 2.270
R-factor	0.2005
Rwork	0.199
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eji
RMSD bond length	0.003
RMSD bond angle	0.735
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.850	2.340
High resolution limit [Å]	2.270	2.270
Number of reflections	26101	2337
<I/σ(I)>	14.4
Completeness [%]	99.1	97.7
Redundancy	3.9	3.9
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290.15	10% PEG 3350, 0.1 M sodium malonate pH 5.0