8DVX
Structure of acetylated Pig somatic Cytochrome c (Aly39) at 1.5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.12713 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.662, 54.559, 61.782 |
| Unit cell angles | 65.97, 87.40, 84.07 |
Refinement procedure
| Resolution | 30.380 - 1.500 |
| R-factor | 0.1716 |
| Rwork | 0.171 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c0z |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.578 |
| Data reduction software | AutoProcess |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.610 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.078 | 0.532 |
| Rmeas | 0.091 | 0.641 |
| Rpim | 0.047 | 0.352 |
| Number of reflections | 110978 | 7704 |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 86.9 | 80.6 |
| Redundancy | 3.5 | 3.1 |
| CC(1/2) | 0.997 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | JBS3A2: 10% PEG 4K, 20% iso propanol, 15% PEG 4K, 2.5 mM Potassium Ferricyanide. |
