8DVN
Crystal structure of LRP6 E3E4 in complex with disulfide constrained peptide E3.10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-12-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000030 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.097, 116.783, 127.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.330 - 2.530 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a0p |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20rc3-4406_final) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.330 | 2.610 |
High resolution limit [Å] | 2.530 | 2.530 |
Rpim | 0.039 | 0.810 |
Number of reflections | 34563 | 22437 |
<I/σ(I)> | 15.13 | |
Completeness [%] | 99.9 | 99.88 |
Redundancy | 6.7 | 6.6 |
CC(1/2) | 0.999 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350, 0.2 M KSCN |