8DVC
Receptor ShHTL5 from Striga hermonthica in complex with strigolactone agonist GR24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97903 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.269, 352.967, 124.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.801 - 2.638 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cbk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_3092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.680 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.084 | 0.889 |
| Rpim | 0.040 | 0.457 |
| Number of reflections | 61856 | 3168 |
| <I/σ(I)> | 13.36 | 0.89 |
| Completeness [%] | 96.1 | 99.6 |
| Redundancy | 5.1 | 4.5 |
| CC(1/2) | 0.536 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 2 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, 8 mM GR24, 1% DMSO, 1% isopropanol, cryoprotectant: ethylene glycol |
