8DT6
Crystal Structure of DNA Polymerase III beta subunit from Elizabethkingia anophelis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-09-16 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9787 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.410, 88.010, 100.240 |
Unit cell angles | 90.00, 95.75, 90.00 |
Refinement procedure
Resolution | 40.260 - 2.350 |
R-factor | 0.1773 |
Rwork | 0.176 |
R-free | 0.21830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.260 | 40.260 | 2.410 |
High resolution limit [Å] | 2.350 | 10.510 | 2.350 |
Rmerge | 0.052 | 0.022 | 0.548 |
Rmeas | 0.059 | 0.025 | 0.626 |
Total number of observations | 280947 | ||
Number of reflections | 66682 | 768 | 4922 |
<I/σ(I)> | 18.5 | 48.97 | 2.73 |
Completeness [%] | 99.8 | 96.2 | 99.8 |
Redundancy | 4.213 | 3.763 | 4.236 |
CC(1/2) | 0.999 | 0.999 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 287 | [Target: ElanA.17987.a.AE1.PS38659 - 28.82 mg/mL] [Barcode: 321211c7] [Pin: mjs7-5] [Crystallization: sparse screen - MCSG1 C7 - 100 mM Tris:HCl pH 8.5, 200 mM Calcium chloride, 25% (w/v) PEG 4000, 14C, 0.2:0.2 drop ratio] [Cryo: 15% EG] |