8DRJ
Apo B2 dimer (H60/H100/H104) formed in the presence of Cu(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.23981 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.320, 48.020, 45.120 |
Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
Resolution | 44.860 - 2.400 |
R-factor | 0.2008 |
Rwork | 0.191 |
R-free | 0.28310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bc5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.032 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.860 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.039 | 0.083 |
Rmeas | 0.055 | 0.117 |
Rpim | 0.039 | 0.083 |
Number of reflections | 7510 | 1383 |
<I/σ(I)> | 11.32 | 6.72 |
Completeness [%] | 99.4 | 99.32 |
Redundancy | 1.9 | 1.9 |
CC(1/2) | 0.996 | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG3350 25%, (NH4)2SO4 200 mM, Tris 100 mM, CuCl2 4 mM |