8DR3
Closed state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2) with NTD
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.2 |
| Software | cryoSPARC,SerialEM,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
