8DQO

Crystal structure of Arabidopsis thaliana COSY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	57.411, 57.903, 270.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 1.900
R-factor	0.1916
Rwork	0.190
R-free	0.23017
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g0b
RMSD bond length	0.010
RMSD bond angle	1.326
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.720	1.968
High resolution limit [Å]	1.900	1.900
Number of reflections	71357	6352
<I/σ(I)>	13.42
Completeness [%]	98.7	89.64
Redundancy	1.1	1.1
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	50 mM calcium acetate, 0.1 M sodium cacodylate pH 6.5, 20% glycerol