8DQ6
Structure of A. thaliana MIF/D-DT-like protein-1 (MDL1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 65 |
Unit cell lengths | 66.737, 66.737, 132.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.530 - 1.560 |
R-factor | 0.1833 |
Rwork | 0.182 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kqx |
RMSD bond length | 0.006 |
RMSD bond angle | 0.824 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18_3845: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.530 | 1.620 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.052 | 0.990 |
Rmeas | 0.054 | |
Rpim | 0.012 | 0.236 |
Number of reflections | 46283 | 4257 |
<I/σ(I)> | 31.45 | |
Completeness [%] | 97.5 | 90.4 |
Redundancy | 20 | |
CC(1/2) | 1.000 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM Tris, pH 8.0, 0.2 M calcium acetate, 26% PEG8000, 30% ethylene glycol |