8DPE
Crystal structure of ATP-dependent RNA helicase DDX42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.917, 73.268, 134.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.147 - 1.531 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nho |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.188 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 3.300 | 1.530 |
| Rmerge | 0.056 | 0.029 | 0.838 |
| Rmeas | 0.062 | 0.032 | 0.926 |
| Rpim | 0.025 | 0.013 | 0.386 |
| Total number of observations | 384934 | ||
| Number of reflections | 63581 | 6809 | 6124 |
| <I/σ(I)> | 8.7 | ||
| Completeness [%] | 98.4 | 99.7 | 96.2 |
| Redundancy | 6.1 | 5.9 | 5.4 |
| CC(1/2) | 0.998 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 1 uL 8 mg/mL DDX42 (in 25 mM Tris, pH 7.5, 300 mM sodium chloride, 1 mM TCEP) + 1 uL reservoir solution (6-10% PEG8000, 0.1 M Tris, pH 8.5-9.0) |
