8DP9
Crystal structure of the monomeric AvrM14-B Nudix hydrolase effector from Melampsora lini
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 80.059, 80.059, 293.949 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.840 - 1.760 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AvrM14-A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.993 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (1.19_4092) |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.990 | 1.839 |
| High resolution limit [Å] | 1.760 | 1.761 |
| Rmerge | 0.068 | 0.763 |
| Rmeas | 0.070 | 0.779 |
| Rpim | 0.015 | 0.159 |
| Number of reflections | 56341 | 5485 |
| <I/σ(I)> | 31.89 | 4.18 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 23 | 23.4 |
| CC(1/2) | 1.000 | 0.941 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.8 M Ammonium sulphate 0.1 M Sodium acetate pH 4.2 - 4.4 0.1 M Magnesium chloride |
