8DP9

Crystal structure of the monomeric AvrM14-B Nudix hydrolase effector from Melampsora lini

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 61 2 2
Unit cell lengths	80.059, 80.059, 293.949
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.840 - 1.760
R-factor	0.1787
Rwork	0.177
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AvrM14-A
RMSD bond length	0.009
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (1.19_4092)
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	1.839
High resolution limit [Å]	1.760	1.761
Rmerge	0.068	0.763
Rmeas	0.070	0.779
Rpim	0.015	0.159
Number of reflections	56341	5485
<I/σ(I)>	31.89	4.18
Completeness [%]	99.9	99
Redundancy	23	23.4
CC(1/2)	1.000	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.8 M Ammonium sulphate 0.1 M Sodium acetate pH 4.2 - 4.4 0.1 M Magnesium chloride