8DP7
Structure of Helicobacter pylori EgtU bound to EGT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 134.716, 137.751, 190.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.540 - 3.350 |
| Rwork | 0.231 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8dp6 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.540 | 39.540 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rpim | 0.420 | 3.422 |
| Number of reflections | 25843 | 4620 |
| <I/σ(I)> | 4.3 | 1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.854 | 0.119 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, 22% PEG4000 |
