8DP4

Beta-lactamase CTX-M-14 T235A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-02-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11
Spacegroup name	P 41 21 2
Unit cell lengths	41.960, 41.960, 260.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 1.402
R-factor	0.1627
Rwork	0.162
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ylt
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	1.452
High resolution limit [Å]	1.402	1.402
Rmeas	0.113	0.361
Number of reflections	46454	4563
<I/σ(I)>	9.4
Completeness [%]	97.9	98.87
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M SPG buffer pH 6, 25% (w/v) PEG 1500