8DO8

Crystal structure ATG9 HDIR in complex with the ATG13:ATG101 HORMA dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	45.453, 139.860, 147.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.410 - 2.410
R-factor	0.2054
Rwork	0.204
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c50
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.410	2.496
High resolution limit [Å]	2.410	2.410
Rmerge	0.095	1.279
Rpim		0.530
Number of reflections	37256	3659
<I/σ(I)>	14.55	1.31
Completeness [%]	99.8	99.89
Redundancy	6.6	6.8
CC(1/2)	0.999	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		273	0.1 M HEPES pH 7.5, 0.2 M NaCl, 12% PEG8000