8DO8
Crystal structure ATG9 HDIR in complex with the ATG13:ATG101 HORMA dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.453, 139.860, 147.595 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.410 - 2.410 |
R-factor | 0.2054 |
Rwork | 0.204 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c50 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.410 | 2.496 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.095 | 1.279 |
Rpim | 0.530 | |
Number of reflections | 37256 | 3659 |
<I/σ(I)> | 14.55 | 1.31 |
Completeness [%] | 99.8 | 99.89 |
Redundancy | 6.6 | 6.8 |
CC(1/2) | 0.999 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 273 | 0.1 M HEPES pH 7.5, 0.2 M NaCl, 12% PEG8000 |