8DN7

The crystal structure of the Pisum sativum Toc75 POTRA domains in complex with fab ax9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	P 1
Unit cell lengths	43.212, 43.430, 169.606
Unit cell angles	94.20, 94.21, 104.44

Refinement procedure

Resolution	41.660 - 2.000
R-factor	0.2122
Rwork	0.211
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bjz 5uay
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmeas	0.148
Rpim	0.082	0.668
Number of reflections	69080	7224
<I/σ(I)>	11.58
Completeness [%]	85.9	88.6
Redundancy	3	3
CC(1/2)	0.992	0.275

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M Sodium Hepes pH 7, 18% PEG 12,000